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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Signal. 2015 Feb 26;27(6):1159–1167. doi: 10.1016/j.cellsig.2015.02.020

Figure 8. EHT 1864 had no effect on INS-1 832/13 cell morphology.

Figure 8

INS-1 832/13 cells were cultured overnight with RPMI (2.5 mM glucose and 2.5% fetal bovine serum) in the presence of diluent (de-ionized water) or Simvastatin (30 μM). The cells were also incubated with EHT 1864 (10 μM) for 1 h after overnight incubation in RPMI with 2.5 mM glucose and 2.5% fetal bovine serum. The cells were then visualized under the microscope to observe changes in morphology. Alterations in cell morphology were quantified by counting cells with morphological abnormalities (rounded-up cells), in the selected areas (0.5 cm2) and expressed as mean ± SEM (n=6).