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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Signal. 2015 Feb 26;27(6):1159–1167. doi: 10.1016/j.cellsig.2015.02.020

Figure 9. EHT 1864 potentiates KSIS in INS-1 832/13 cells.

Figure 9

INS-1 832/13 cells were cultured in RPMI media overnight in the presence of 2.5 mM glucose and 2.5% fetal bovine serum. After pre-incubation with EHT 1864 (0–10 μM) for 1h, cells were incubated in the presence of low glucose (2.5 mM) or KCl (40 mM) in the continuous absence or presence of EHT 1864 for 30 min at 37°C. The amount of insulin released was quantified by ELISA. The data was expressed as ng/ml of insulin released ± SEM and * p < 0.05 vs. 2.5 mM glucose, and # p < 0.05 vs. 40 mM KCl alone or in presence of 5 μM EHT 1864.