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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Nat Genet. 2015 Mar 2;47(4):338–344. doi: 10.1038/ng.3229

Figure 5.

Figure 5

Molecular interaction among SEC, cohesin and RNAP2. Results of immunoprecipitation-Western blot. (a) Protein interaction between SEC and cohesin. STAG1 (SA1) interacts with various SEC components including AFF4, ELL2, Cyclin T1 and CDK9. Immunoprecipitation with STAG2 (SA2) did not show such interaction with SEC. Addition of DRB slightly increased the amount of SEC components interacting with SA1. (b) Protein interaction between cohesin and RNAP2. STAG1 (SA1) interacts with various forms of RNAP2. Addition of DRB (D) and flavopiridol (F) decreased the amount of RNAP2 Ser2ph and Ser5ph precipitated with STAG1. RNAP2 was not immunoprecipitated with STAG2 (SA2) antibody.