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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Phytochemistry. 2014 Nov 4;112:151–159. doi: 10.1016/j.phytochem.2014.09.015

Figure 4. Peroxidase activity assay of E. coli expressing CG5-6xHis using ABTS.

Figure 4

Three individual clones of E. coli expressing CG5-6xHis (CG5_1, CG5_2, and CG5_3) were tested for peroxidase activity using the ABTS assay (Everdeen et al. 1988). Insoluble proteins from E. coli transformed with the empty-vector were assayed as a background control. Activities for each sample were recorded in triplicate, averaged, then normalized to folding buffer-only controls (=1). Error bars represent standard deviations of triplicate measurements.