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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Phytochemistry. 2014 Nov 4;112:151–159. doi: 10.1016/j.phytochem.2014.09.015

Figure 6. Initial crosslinking rates of Tomato pI4.6 EP and CG5-6xHis.

Figure 6

Endogenous tomato P1 and synthetic P3 analog (YK20) extensins were used as substrates for in vitro crosslinking reactions to calculate initial rates (μg of extensin crosslinked/sec/ng peroxidase). Crosslinking reactions were stopped after two-minutes of incubation to facilitate rate calculations for each substrate (Everdeen et al., 1988; Held et al., 2004; Schnabelrauch et al., 1996). Initial rates were calculated as described earlier using the first order rate equation and normalized to the amount of peroxidase (ng POX) per reaction (Everdeen et al. 1988). Error bars represent the standard deviation of the mean crosslinking rates of triplicate reactions. FK9 (not shown) showed no crosslinking whatsoever.