Table 2.

Spectroscopic and kinetic values for de novo designed NiR mimics based on the TRI scaffold. All values are reported for pH 5.8.

peptide	Δcharge	d-d λmax (ε/M−1cm−1)	reduction potential	CuI Kd, [M−1]	CuII Kd, [M−1]	rate [M−1min−1]	pKaE	pKaW
TRI-H	0	644 (143)	402±8	(3.1±0.7) × 10−12	(4.0±0.8) × 10−8	(2.2±0.2) × 10−6		8.53±0.02
TRI-EHE27K	0	671 (80)	600±10	(1.4±0.6) × 10−14	(3±1) × 10−7	(1.1±0.7) × 10−6		9.6±0.2
TRI-EHE27Q	−3		560±10	(2±1) × 10−14	(1.3±0.3) × 10−7	(1.3±0.9) × 10−6
TRI-EH	−6	659 (110)	590±10	(4.2±0.2) × 10−14	(7±3) × 10−7	(2.0±0.3) × 10−6	6.33±0.04	9.86±0.05
TRI-EHK24Q	−9		490±20	(5±3) × 10−13	(2±1) × 10−7	(3.8±0.8) × 10−6
TRI-EHK24E	−12	665 (101)	520±12	(3.52±0.09) × 10−14	(5±2) × 10−7	(3.6±0.6) × 10−6	6.76±0.06	9.8±0.2

From ref [25]