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. 2015 Apr;185(4):1104–1113. doi: 10.1016/j.ajpath.2014.12.019

Figure 4.

Figure 4

RNA sequencing analysis. A: Venn diagram depicting the differentially expressed genes (DEGs) common and/or unique to Ptenf/f, Pik3caE545K, and Ptenf/f;Pik3caE545K. B: Real-time quantitative PCR analysis to validate expression of DEGs identified by RNA sequencing. Graph depicts fold change in expression of Axin2, Hspa1a, and Cdkn2a in Ptenf/f, Pik3caE545K, and Ptenf/f;Pik3caE545K cells as compared to green fluorescent protein (GFP)–treated cells. Means ± SEM are summarized. N = number of independent experiments performed for each gene.