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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Feb 20;1854(5):469–475. doi: 10.1016/j.bbapap.2015.02.007

Fig. 3.

Fig. 3

Western blot analysis of PAFAH-II constructs resolved by native PAGE and SDS PAGE. (A) Western blot analysis resolved by native PAGE: lane 1: WT-PAFAH-II-YFP-His, lane 2: G2A mutant-PAFAH-II-YFP-His, and blotted with GFP specific antibodies. WT-PAFAH-II displays monomer and dimer bands, while the myristoyl mutant resolves as a monomer. (B) Western blot analysis resolved by SDS PAGE: molecular weight marker lane (kDa) on left, lane 1: WT-PAFAH-II-YFP-His, lane 2: G2A-PAFAH-II-YFP-His, and blotted with GFP specific antibodies. The wild type and G2A-PAFAH-II fusions with YFP run at their expected molecular weight of 77 kDa.