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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Feb 20;1854(5):469–475. doi: 10.1016/j.bbapap.2015.02.007

Fig. 7.

Fig. 7

Confocal microscopy images of HEK293 cells expressing PAFAH-II WT, G2A and hydrophobic patch mutant fusions with fluorescent protein. Representative images showing WT-PAFAH-II-YFP, G2A-PAFAH-II-YFP, or Triple-mutant-PAFAH-II-CFP cellular locations. WT-PAFAH-II-YFP was evenly distributed in the cytoplasm and cytoplasmic membranes (shown by white arrows), while PAFAH-II levels were notably reduced inside the nucleus. In contrast, the G2A myristoyl mutant and the hydrophobic patch triple mutant (L327S/I328S/F331S) were evenly distributed in the cytoplasm and nucleus. This image was adapted from a figure of our previous work [12].