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. Author manuscript; available in PMC: 2016 Mar 26.
Published in final edited form as: Protein Expr Purif. 2014 Sep 26;104:92–102. doi: 10.1016/j.pep.2014.09.012

Figure 6. Co-expression and purification of MmPtcT1 with Shh.

Figure 6

Sf9 cells were co-infected with baculoviruses driving expression of MmPtcT1 along with ShhFL/Hhat. (A) MmPtcT1 was purified and visualized with Coomassie-stained SDS-PAGE. The presence of co-purifying Shh followed by Western blot. Two major Shh bands are observed: one corresponding to the molecular weight of the unprocessed Shh (ShhFL), and one corresponding to the expected molecular weight of ShhNp. (B) The resulting co-purified proteins were subjected to Superose6 SEC. The fractions were analyzed by Coomassie-stained SDS-PAGE for MmPtcT1 detection, and by Western blot for Shh.