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. Author manuscript; available in PMC: 2016 Mar 12.
Published in final edited form as: Cell. 2015 Mar 5;160(6):1246–1260. doi: 10.1016/j.cell.2015.02.038

Figure 1.

Figure 1

(A) Schematic representation of the loss-of-function metastasis screen using the mouse genome-scale CRISPR/Cas9 knock-out library (mGeCKOa).

(B) Representative hematoxylin and eosin (H&E) stains of primary tumor from Nu/Nu mice subcutaneously transplanted with a Cas9-GFP KrasG12D/+;p53−/−;Dicer1+/− (KPD) NSCLC cell line, untransduced or transduced mGeCKOa lentiviral library. Scale bar: 200 μm.

(C) Primary tumor growth curve of Nu/Nu mice transplanted with untransduced cells (n = 3 mice) or mGeCKOa-transduced Cas9-GFP KPD cells (n = 9 mice).

(D) Micro-CT 3D reconstruction of the lungs of representative mice transplanted with control (untransduced) and mGeCKOa-transduced (mGeCKOa) cell pools. Lung metastases were identified and traced in each 2D section (green).

(E) Percent of lobes with metastases visible after dissection under a fluorescence stereoscope, in Nu/Nu mice transplanted with untransduced Cas9-GFP KPD cells (n = 3 mice), or mGeCKOa-transduced Cas9-GFP KPD cells with three independent infection replicate experiments (R1, R2 and R3, n = 3 mice per replicate).

(F) Representative H&E stains from various organs of Nu/Nu mice subcutaneously transplanted with untransduced and mGeCKOa-transduced Cas9-GFP KPD cells. Yellow arrow indicates a lung metastasis. Scale bar: 40 μm.

(See also: Figure S1)