Table 1.
List of primers used for PCR detailing accession number, primer sequence, annealing temperature and expected amplification product size
| GENE | Accession Number | PRIMERS | Tm (°C) | Amplification product |
|---|---|---|---|---|
| Staufen (STAU) | NM_017454.2 | FOR 5’- cacctccgtgtttggtcttt -3’ REV 5’- ggtcacgctgagtaggaagc -3’ |
60 | 183 bp |
| Calreticulin (CALR) | NM_004343.3 | FOR 5’- tctcagttccggcaagttct -3’ REV 5’- tctgagtctccgtgcatgtc -3’ |
60 | 231 bp |
| Homo sapiens heat shock 70 kDa protein 5 (HSPA5) | NM_005347.4 | FOR 5’- tagcgtatggtgctgctgtc -3’ REV 5’- tttgtcaggggtctttcacc -3’ |
60 | 241 bp |
| Nestin (NES) | NM_006617.1 | FOR 5’- aacagcgacggaggtctcta -3’ REV 5’- ttctcttgtcccgcagactt -3’ |
60 | 220 bp |
| Homo sapiens ribosomal protein S28 (RPS28) | NM_001031.4 | FOR 5’- tccatcatccgcaatgtaaa -3’ REV 5’- tgtgacagaccattcccatc -3’ |
60 | 157 bp |