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. 2015 Jan 12;4(3):415–425. doi: 10.1002/cam4.369

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© 2015 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Forced PPL expression induced desmosome-like structures. (A) TEM images were obtained from normal human esophageal mucosa (normal) and mock-transfected or PPL-transfected KTSE270 cells at 10,000× or 30,000× magnification. Desmosomes were frequently found in normal tissues and PPL-transfected cells (arrows). In the images of the mock-transfected cells taken at 10,000× magnification, the arrow indicates adhesion plaque-like structures, which was not identified as a desmosome at higher magnification. (B) Expression of envoplakin (EVPL) in mock- or PPL- transfected KYSE270 cells. EVPL mRNA levels were shown as fold expression of the levels to normal esophageal mucosa (average of 13 mucosa = 1). Data are shown as mean + SD of three assays. (C) Expression of envoplakin (EVPL) in ESCC tissues with paired normal mucosa. PPL, periplakin; ESCC, esophageal squamous cell carcinoma.