Figure 7.

Validation of eboIZN21 as a phage display target. Clonal phage expressing an ebolavirus C-peptide (eboC24) were incubated with biotinylated eboIZN21 bound to streptavidin magnetic beads (solid-phase conditions). Negative target controls include the binding site mutant, eboIZN21(D2), and magnetic beads with no target (NT). Binding of M13KE (phage with no peptide clone) to all targets was also assayed. The fraction of phage bound is reported. Error bars represent standard error for triplicate experiments.