Figure 8.

Comparing the two ebolavirus N-trimer mimics as phage display targets. (A) Phage background binding is greater to eboIZN39IQ than to eboIZN21. Phage binding assay showing M13KE control phage binding to biotinylated eboIZN39IQ and eboIZN21 under both solid-phase (left) and solution-phase (right) conditions. Magnetic beads with no target (NT) were used as a negative control. The fraction of phage bound is reported. Error bars represent the range for duplicate experiments (solid phase) and standard error for four or more replicates (solution phase). (B) High stringency solution-phase binding shows an affinity difference for the specific binding of eboC24 to the two N-trimer mimics. Clonal phage expressing eboC24 were incubated with biotinylated N-trimer in solution followed by capture via magnetic streptavidin beads. Negative target controls include the binding site mutants and magnetic beads with no target (NT). For NT, error bars represent standard error across triplicate experiments. The remaining error bars represent the range for duplicate experiments.