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Limited chymotryptic digestion of R67 DHFR DNM (upper panel) and DSM variant 3S (lower panel), observed by tricine SDS PAGE. R67 DHFR DNM or DSM 3S were digested with chymotrypsin (1:100 chymotrypsin : R67 DHFR) for 2–24 h at 4°C, as indicated above each lane. Chy: chymotrypsin reference; M: molecular weight markers. Arrows on the right indicate the bands corresponding to the monomeric and dimeric species.
