Table II.
Kinetic Parameters for the Non-Mutated DNM Dimer and Selected Mutated DSM Dimer Variants
| Residues 66–69 in N-terminal protomer | kcat (s−1) | KMDHF (µM) | KMNADPH (µM) | kcat/KMNADPH (s−1 µM−1) | kcat/KMDHF (s−1 µM−1) |
|---|---|---|---|---|---|
| DNM: VQIY (native) | 0.28 ± 0.03 | 13 ± 4 | 13 ± 5 | 0.02 | 0.02 |
| DSM: TENH (3S) | 0.18 ± 0.01 | 28 ± 4 | 19 ± 4 | 0.01 | 0.007 |
| ANTF (4S) | 0.28 ± 0.02 | 51 ± 8 | 44 ± 6 | 0.006 | 0.005 |
| TEFL (5S) | 0.03 ± 0.01 | 35 ± 23 | ND | — | 0.001 |
| INSF (9S) | 0.06 ± 0.01 | 16 ± 4 | >200 | < 0.0003 | 0.004 |
| TEFY (12S) | 0.12 ± 0.01 | 18 ± 5 | 84 ± 20 | 0.001 | 0.007 |
| IKYH (13S) | 0.050± 0.004 | 15 ± 4 | 13 ± 2 | 0.004 | 0.003 |
| AEFL (25S) | ND | ND | ND | — | — |
| HEYF (27S) | 0.10 ± 0.01 | 8.5 ± 3.0 | 23 ± 7 | 0.005 | 0.012 |
| NKNY (30S) | 0.11 ± 0.01 | 13 ± 4 | 16 ± 5 | 0.007 | 0.009 |