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Recovery of Enzymatic Activity Following High-temperature Incubation
| Condition | DNM (%) | 3S (%) | 9S (%) | 12S (%) | 30S (%) |
|---|---|---|---|---|---|
| A: 95°C, 90 min; RT, 1 min | 11 | 18 | 18 | 26 | 17 |
| B: 95°C, 90 min; RT, 45 min | 23 | 72 | 72 | 46 | 47 |
| C: 95°C, 4 min; RT, 1 min | 78 | 95 | 90 | 50 | 11 |
| D: 95°C, 4 min; RT, 45 min | 71 | >99 | 90 | 53 | 70 |
Samples were incubated at 95°C then at room temperature for the periods indicated, then activity was measured. Activity is expressed as percent of activity for that variant prior to incubation.
