Figure 3.

Template side-chain variability. χ₁ dihedral angles for rotamers found at each design position in the crystal (XTAL) and NMR structures prior to (−) or following energy minimization (+) are shown as black lines. χ₁ dihedral angles for rotamers found at each design position in ROM templates grouped by the stability of their seed sequence are shown as sections of pie charts colored in black. These sections represent the range of values that the χ₁ dihedral can adopt in ROM templates. Dihedral values are binned into one of three general conformations: gauche⁺ (light gray), trans (medium gray), and gauche⁻ (dark gray). The number of ROM templates sharing the same general rotamer conformation at each design position is indicated next to each section.