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Michaelis-Menten plot of retro-aldol activity catalyzed by CaM (red squares), cCaM (purple diamonds), cCaMWN (blue circles), CaM F92K (green triangles), CaM L105K (black squares) and Ac-cCaMWN (orange triangles). Conditions: 20 mM HEPES (pH 7); 100 mM NaCl; 10 mM CaCl2; substrate final concentration: 0.1–1.5 mM; 40 μM protein.
