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Aggregation of α-synuclein in vitro. A, B, Recombinant wild-type α-synuclein or mutant α-synuclein unable to bind to liposomes were incubated in buffer (left panels) or in the presence of charged liposomes (composition: 30% PS, 70% PC; right panels) at 37°C and 300 rpm. At the indicated time points, aggregation of α-synuclein was assessed by light microscopy.
