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. 2015 Apr 1;35(13):5260–5270. doi: 10.1523/JNEUROSCI.4365-14.2015

Figure 3.

Figure 3.

Role of the PDZ ligand of EAAT2b in cell-surface localization. A, Illustration of the mutants and chimeras used to investigate membrane localization of EAAT2b. B, MDCK cells transiently transfected with EGFP-tagged mutants and chimeras were quantified for their surface membrane expression. Addition of the PDZ ligand of EAAT2b stabilizes EAAT2a at the membrane. Deletion of the PDZ ligand of EAAT2b destabilizes the surface localization of the isoform. *p < 0.05; **p < 0.01 to EAAT2a; +p < 0.01 to EAAT2b by one-way ANOVA (n = 5). Error bars indicate SEM. C, Quantitation of polarization of EAAT2a, EAATb, and the chimeras by biotinylation indicates that the PDZ ligand also dictates basolateral targeting of the EAAT2b isoform. *p < 0.0001 by two-way ANOVA (n = 5). Error bars indicate SEM.