Effects of LRRK2 kinase inhibition in primary microglia. A–F, ICC of primary murine microglia for LRRK2 (green), CD11b (red), and DAPI (blue) after saline (A–C) or Tat (D–F) treatment with or without LRRK-IN-1 and GSK2578215A (40×). G, Western blot of pS935–LRRK2, total LRRK2, and β-actin protein levels 6 h after saline or Tat treatment with or without LRRK2-IN-1 and GSK2578215A. H, Tat-induced pS935–LRRK2 is significantly decreased by LRRK2-IN-1 and GSK2578215A (**p < 0.01, one-way ANOVA with Newman–Keuls post hoc test).