Figure 6.

Synaptic vesicle puncta are preserved in Tat-injected LRRK2 KO mice. IHC for synapsin-1 (red; A–E) and VGluT (red; F–J) was performed, and two 60× images were taken at the injection site per animal and analyzed using the Velocity software program, which revealed that Tat-injected WT mice have significantly fewer synaptic puncta (B, E, G, J) compared with Tat-injected LRRK2 KO mice (D, I) and saline control animals (A, C, F, H) (*p < 0.05, **p < 0.01, one-way ANOVA with Newman–Keuls post hoc test). Scale bars, 11 μm.