Figure 7.

LRRK2 KO mice exhibit decreased neutrophil infiltration 7 d after Tat injection. A–G, IHC for 7/4 (green) and DAPI (blue) was performed, and one 10× image of the injection site was counted manually for 7/4-positive cells per animal per treatment and showed a significant decrease in the number of 7/4-positive neutrophils in Tat-injected LRRK2 KO mice (E, 10×; F, 60×; G, ** p < 0.01) compared with Tat-injected WT mice (B, 10×; C, 60×), whereas very few neutrophils were observed in saline control animals (A, 10×; D, 10×). H, Western blot of total brain homogenate from WT and LRRK2 KO mice for total LRRK2 protein levels (C, F, white arrows depict multi-lobed nuclei; one-way ANOVA with Newman–Keuls post hoc test). Scale bars: A, B, D, E, 60 μm; C, F, 11 μm.