Figure 9.

LRRK2 KO normalizes microglia morphology in Tat-injected mice. A–P, IHC for single-channel Iba1 (green; A, E, I, M, 20×; D, H, L, P, 60×), MAP2 (red; B, F, J, N, 20×), and merged images with DAPI (blue; C, G, K, O, 20×) was performed. Q–R, Images as 20× taken at the injection were labeled and skeletonized using Amira software and microglia from Tat-injected LRRK2 KO mice (M–P) have significantly more processes (Q) and longer processes (R) compared with Tat-injected WT mice (I–L) (***p < 0.001, one-way ANOVA with Newman–Keuls post hoc test). Scale bars: A–C, E–G, I–K, M–O, 32 μm; D, H, L, P, 11 μm.