Figure 2.

Catalytic activity of TMPAP prevents accumulation of ATP within the intracellular vesicular compartments. A, Left, Fluorescent image of astrocytes in the same cell culture showing that TMPAP expression prevents labeling of intracellular vesicular compartments with MANT-ATP. Right, Colocalization line profile following the arrow (red), illustrating intensity of fluorescence of TMPAP-EGFP (green) and MANT-ATP (blue). 1, Cell transduced to express TMPAP. 2, Cell that was not transduced. B, Left, Fluorescent image of a cultured astrocyte transduced to express EGFP displaying abundant labeling of putative ATP-containing vesicles with MANT-ATP. Right, Colocalization line profile following the arrow (red), illustrating intensity of fluorescence of EGFP (green) and MANT-ATP (blue). C, Left, Fluorescent image of cultured astrocytes transduced to express TMPAP-EGFP, showing absence of MANT-ATP vesicular labeling. Right, Colocalization line profile following the arrow (red), illustrating intensity of fluorescence of TMPAP-EGFP (green) and MANT-ATP (blue).