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CE analysis of His6-RMDPa reactions. His6-RMD converts the product of the His6-GMD-catalyzed reaction, GDP-6-deoxy-d-lyxo-hexos-4-ulose, to GDP-d-Rha, in the presence of NADPH. Traces: (A) standard, GDP-d-Man; (B) the product of His6-GMD incubation with GDP-d-Man after removal of His6-GMD by filtration; (C) the His6-GMD product shown in (B), after subsequent incubation with His6-RMDPa and NADPH.
