Table 1.
NMR data for sugar nucleotide metabolites in the GDP-d-Rha pathway of P. aeruginosa. Resonances were referenced to an internal acetone standard at δH = 2.225 p.p.m. and δC = 31.07 p.p.m.
|
1H and 13C chemical shifts [δ (p.p.m.)], and proton coupling constants (JH,H (Hz)] |
|||||||
|---|---|---|---|---|---|---|---|
| Compound | H1 C1 J1,2 | H2 C2 J2,3 | H3 C3 J3,4 | H4 C4 J4,5 | H5 C5 J5,6 | H6/6′ C6 | |
| GDP-α-d-mannose (A) | δ H | 5.51 | 4.05 | 3.92 | 3.68 | 3.84 | 3.75/3.85 |
| δ C | 97.1 | 70.9 | 69.6 | 67.2 | 74.1 | 61.6 | |
| 3 J H,H | 1.8 | 3.4 | 10.3 | 10.0 | |||
| 3 J H,P | 7.9 | ||||||
| GDP-α-d-6-deoxy-d-lyxo-hexos-4-ulose (B) | δ H | 5.58 | 4.46 | 4.84 | 4.68 | 1.22 | |
| δ C | 96.3 | 76.0 | 73.1 | 208.8 | 71.9 | 13.4 | |
| 3 J H,H | 2.2 | 3.8 | 6.5 | 6.5 | |||
| 3 J H,P | 7.6 | ||||||
| gem-Diol form of GDP-α-d-6-deoxy-d-lyxo-hexos-4-ulose (C) | δ H | 5.45 | 4.01 | 3.93 | 4.06 | 1.20 | |
| δ C | 97.4 | 71.5 | 69.6 | 94.0 | 71.0 | 12.3 | |
| 3 J H,H | 1.8 | 3.5 | 6.5 | 6.5 | |||
| 3 J H,P | 7.6 | ||||||
| GDP-α-d-rhamnose (D) | δ H | 5.43 | 4.03 | 3.86 | 3.42 | 3.89 | 1.25 |
| δ C | 97.2 | 71.2 | 70.4 | 72.8 | 70.4 | 17.6 | |
| 3 J H,H | 1.2 | 3.5 | 9.7 | 9.8 | 6.1 | ||
| 3 J H,P | 7.6 | ||||||