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. 2015 Feb 19;43(6):3272–3285. doi: 10.1093/nar/gkv078

Figure 2.

Figure 2.

RNAP concentration dependence of promoter opening. (A) Fluorescence enhancement as a function of time for increasing concentrations of EcoRNAP (0–238 nM). (B) Fluorescence enhancement as a function of time for increasing concentrations of MboRNAP (0–600 nM). (C) Equilibrium fluorescence fold change for both EcoRNAP and MboRNAP as a function of polymerase concentration. Fits (solid lines) of the data allow the extraction of concentrations of half-maximal effect (23 ± 5 nM and 212 ± 43 nM) and saturated fluorescence enhancements (1.44 and 0.28) for EcoRNAP and MboRNAP, respectively.