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CarD increases open-complex formation with MboRNAP. (A) Fluorescence enhancement as a function of time for 225 nM MboRNAP with increasing concentrations of CarD (0–5.55 μM) at 25°C. (B) Equilibrium values of fluorescence enhancement are plotted as a function of CarD concentration. A fit (solid line) of the data reveals a concentration of half-maximal effect Keff = 77 ± 35 nM and an amplitude of 6.2-fold over 225 nM MboRNAP alone.
