Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 19;43(6):3272–3285. doi: 10.1093/nar/gkv078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 6. — A CarD-dependent kinetic model of open-complex formation. (A) The model consists of five states: unbound DNA (R + P), closed complex (RP_c), open complex (RP_o), closed complex bound to CarD (RP_cCarD) and open complex bound to CarD (RP_oCarD). Values indicate the ratio of rate constants between each pair of states in the model. For example, at 250 nM, the forward and reverse rates between R + P and RP_c are equal. Furthermore, the ratio of forward to reverse rates between RP_c and RP_o are 0.125 and 40 in the absence and presence of CarD, respectively. The rates used to generate the figures below can be found in Supplementary Information. (B) Simulations of the kinetic model titrating CarD concentration with 150 nM (black) and 225 nM (red) MboRNAP. (C) Simulations of the kinetic model titrating RNAP concentration with 0 nM (black) and 1 μM (red) CarD.