Figure 1.

U1 snRNP-specific proteins are required for Gem formation. (A and B) HeLa cells stably expressing shRNA against FUS or transfected with siRNAs against U1-70K, U1A, or U1C were used to knock down these proteins. Scrambled shRNA or siRNA was used as a negative control. The knockdowns were analyzed by Western using the indicated antibodies. Tubulin was used as a loading control. (C) IF of the indicated knockdown cells with SMN antibodies (green) was used to detect Gems. DAPI (blue) was used to mark the nucleus. The merged images are shown. Scale bar: 20 μm.