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Influence of mutations in tRNAVal2 sequence on the stability of the termination complexes. (A) Positions where substitutions were inserted. (B) Toe-prints with eRF1+eRF3c+GTP and mutated tRNAVal2 at UAA stop codon. cDNAs corresponding to preTC and TC have the 127 and 125 nt length, respectively. RFU, relative fluorescence unit. The experiments have been replicated at least three times.
