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. 2015 Mar 9;43(6):3332–3343. doi: 10.1093/nar/gkv171

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Influence of the analogs of the tRNA acceptor end on the stability of the termination complexes. (A) Structure of the mini-helix obtained from tRNA^Val(CG). (B) Toe-prints with eRF1, eRF1(AGQ) and eRF3c+GTP and mini-helix obtained from tRNA^Val(CG) at UAA stop codon. (C) Toe-prints with eRF1, eRF1(AGQ) and eRF3c + GTP and cycloheximide at UAA stop codon. cDNAs corresponding to preTC and TC have the 127 and 125 nt length, respectively. RFU, relative fluorescence unit. The experiments have been replicated at least three times.