Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 9;43(6):3344–3357. doi: 10.1093/nar/gkv196

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — USIP-1 is a terminal uridylyl transferase. (A) Schematic representation of the domain structure of STPAP/RBM21/TUT1 (UniProt: STPAP_HUMAN) and MTPAP/PAPD1 (PAPD1_HUMAN) from H. sapiens, and the C. elegans proteins GLD-4 (GLD4_CAEEL) and USIP-1 (Q23652_CAEEL). tm1897 denotes a deletion allele of USIP-1 that leads to a frame shift (striated) and premature termination codon at position 276. C₂H₂ ZF = C₂H₂-type (classical) zinc finger (ZF), RRM = RNA recognition motif, PAD = PAP-associated domain, DXD: Amino acid motif required for catalytic activity where D = aspartic acid and X = any amino acid. (B) Assay to test terminal transferase activity of recombinant USIP-1 and USIP-1^cd. Twenty micrograms of recombinant protein was added to 200 ng of a synthetic 22-nucleotide-long RNA substrate in the presence of 1 mM UTP. The reaction was stopped by addition of Trizol between 0 and 10 min. Shown is a SYBR Gold staining of a 15% urea–polyacrylamide gel (inverted picture). M = Marker. (C) Transferase assay similar to (B) with different nucleotide triphosphates. (D) Autoradiography of transferase activity of recombinant USIP-1 or USIP-1^cd on gel-purified U6 RNA from the indicated sources using radioactively labeled UTP-α-^[32]P.