Figure 2.

Differentiation of induced pluripotent stem cells into induced pluripotent stem cell-derived endothelial cells. (A) An outline of the protocol used for the differentiation of murine induced pluripotent stem cells to induced pluripotent stem cell-derived endothelial cells. At Day 14, induced pluripotent stem cell-derived endothelial cells were stained for CD31 and CD144, and double-positive (CD31⁺/CD144⁺) cells were expanded on 0.2% gelatin-coated plates in EGM-2 medium. (B) Representative brightfield images and positive immunofluorescence of differentiated induced pluripotent stem cell-derived endothelial cells for CD31 and CD144 at 63×. Differentiated induced pluripotent stem cell-derived endothelial cells also exhibited DiI-Ac-LDL uptake.