Figure 1.

Dependence of the reaction times (s) in the hot-plate test 15 min after intravenous injection of 10 mg/kg of a dalargin solution (DAL/SOL) or of dalargin-coated nanoparticles (DAL/NP) on the time of day. Groups of 10–12 DAB/2 mice were tested every 2 h within 24 h; lights on from 07:00 to 19:00. Shown are mean values ± SEM; the solid line represents the cosine fit to the data. (Adapted with permission from [13]. Copyright 1999 Informa Healthcare).