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. 2015 Jan 9;4(1):40–55. doi: 10.3390/cells4010040

Figure 1.

Figure 1

Unambiguous detection of Peripheral Blood Mononuclear Cells (PBMC) positive for Acridine Orange, Propidium Iodide, and Po-Pro-1. PBMC were stained with either Acridine Orange, Propidium Iodide, or Po-Pro-1™ and were imaged at the specified excitation and emission wavelengths. AO-stained (live) cells are detected at 480/520 nm only, PI-stained (dead) cells at (560/620 nm) only, Po-Pro-1 stained (apoptotic) cells at 405/420 nm only. No cross bleeding of signal between the dyes is detectable, providing unambiguous detection of each cell type.