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. 2015 Jan 9;4(1):40–55. doi: 10.3390/cells4010040

Figure 5.

Figure 5

Percentage of live, dead, and apoptotic T cells, and T cell functionality after storing PBMC at 4 °C or 37 °C overnight. PBMC were stored at 4 °C or 37 °C overnight. (A) The numbers of live, dead, and apoptotic cells was determined after overnight storage. (B) PBMC stored overnight at 4 °C (solid bars) and 37 °C (open bars) were resuspended in fresh medium and tested in an IFN-γ ELISPOT assay with different antigens. CMV peptide pool, EBV peptide pool, and Flu peptide pool were used to activate CD8 cells and Mumps and Mosquito antigens to activate CD4 cells.