Skip to main content
. 2015 Jan 29;4(1):71–83. doi: 10.3390/cells4010071

Figure 2.

Figure 2

PBMC were stained with fluorescent Calcein AM dye and the specified numbers of cells were plated into respective wells of a 96-well plate. The cells were visualized on an ImmunoSpot® S6 Ultimate Reader by using 480 nm as the excitation wavelength, and detecting fluorescence with a 520 ± 20 nm emission wavelength filter.