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. 2015 Jan 29;4(1):71–83. doi: 10.3390/cells4010071

Figure 3.

Figure 3

(A,B) PBMC were plated in an ELISPOT assay in serial dilution at the numbers specified, and HCMV pp65 was added to elicit IFN-γ production by the specific CD8+ cells. The number of PBMC added to a (B) 384-well plate was one-third the number in the (A) 96-well plate. The ideal linear function is shown by the superimposed red line. For the 96-well plate, the experimental data approximated linearity (R2 = 0.9782) between 1.0 × 105 cells per well and 1 × 106 cells per well. For the 384-well plate, linearity was approximated (R2 = 0.9823) between 3.3 × 104 PBMC per well and 3.5 × 105 cells per well.