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. 2015 Mar 6;4(1):216–236. doi: 10.3390/biology4010216

Figure 3.

Figure 3

High-resolution MALDI MSI in negative reflector ion mode. MALDI MSI analysis of porcine ovary section acquired at 22 µm spatial resolution. (A) Skyline projection spectrum of molecular species (lipids) in the m/z 200–1200 range; (B) Histological image of ovary section (digital scan at 7200 dpi) overlaid (merge) on molecular reconstruction image (Lipids MSI); (C) Molecular images of three lipids showing preferential localization to follicular fluid (m/z 463.8), to follicular wall cells (m/z 886.5) and to interstitial tissue, mainly outside follicular fluid (m/z 704.9).