Table 1.
Proportion of A549 cells and EC in the different cell cycle phases 24 h after exposure to 1 Gy of alpha particles or 8 h after exposure to 1.5 Gy of proton beam in mono- or co-culture configurations. Cells were fixed, stained with propidium iodide and percentages of cells in sub-G1, G1, S and G2/M phases were determined by flow cytometry. * = irradiated cells.
| Alpha particles | G1 (%) | S (%) | G2/M (%) | |
| Mono-culture | CTL A549 | 38.9 | 48.9 | 12.1 |
| 1 Gy A549* | 38.4 | 22.5 | 39.2 | |
| Co-culture | CTL A549/EC | 51.3 | 40.6 | 8.6 |
| 1 Gy A549*/EC* | 44.9 | 32.2 | 22.9 | |
| 1 Gy A549*/EC | 45.4 | 30.2 | 24.1 | |
| 1 Gy A549/EC* | 50.8 | 44.9 | 4.3 | |
| Proton beam | G1 (%) | S (%) | G2/M (%) | |
| Mono-culture | CTL A549 | 46.4 | 51.2 | 2.3 |
| 1 Gy A549* | 22.9 | 46.7 | 30.4 | |
| Co-culture | CTL A549/EC | 50.3 | 45.2 | 4.5 |
| 1 Gy A549*/EC* | 27.9 | 45.8 | 26.3 | |
| 1 Gy A549*/EC | 36 | 50.5 | 13.4 | |
| 1 Gy A549/EC* | 47.6 | 51.1 | 1.3 | |
| Alpha particles | G1 (%) | S (%) | G2/M (%) | |
| Mono-culture | CTL EC | 78 | 20.5 | 1.6 |
| 1 Gy EC* | 62.1 | 31.4 | 6.6 | |
| Co-culture | CTL A549/EC | 66.4 | 30.8 | 2.8 |
| 1 Gy A549*/EC* | 69.8 | 19.2 | 11 | |
| 1 Gy A549*/EC | 68.1 | 28.9 | 3 | |
| 1 Gy A549/EC* | 66.5 | 28.9 | 4.6 | |
| Proton beam | G1 (%) | S (%) | G2/M (%) | |
| Mono-culture | CTL EC | 72.9 | 23.3 | 3.7 |
| 1 Gy EC* | 68.1 | 24.9 | 7 | |
| Co-culture | CTL A549/EC | 63 | 35.6 | 1.4 |
| 1 Gy A549*/EC* | 56.6 | 38 | 5.5 | |
| 1 Gy A549*/EC | 62.4 | 34.8 | 2.9 | |
| 1 Gy A549/EC* | 57 | 39.8 | 3.23 |