Figure 7.

Suppression of K562 and HEL leukemic cell growth and viability by the recombinant cell-penetrating S5-DBD-PA. S5-DBD-PA protein was added to the culture media of K562 and HEL cells in final concentrations of 0.5, 1, 1.5 µM. For negative control 1.5 µM of the non-specific hTRX scaffold protein construct and the same volumes of protein-solvent (dialysis buffer) and PBS were added. Medium and proteins were replaced daily and cell viability and growth were determined by XTT assay over 5 consecutive days. Results are shown as the percentage of viable cells compared to the PBS control (n = 4; Ø ± SD). Significantly reduced XTT-values in comparison to protein solvent treated cells are indicated. * p < 0.05, ** p < 0.01, *** p < 0.001 (2-way-ANOVA with Bonferroni correction).