Figure 6. Effects of chronic vmPFC HFS on the Golgi measurement of dendritic spine density and immunofluorescence labeling of neurogenesis-related cell function in the hippocampal DG area.
Note: there was an increase in the secondary, but not the primary dendritic spine density of the Golgi-impregnated cells in the DG area of the hippocampus (A–B; scale bar: 10 μm). Representative confocal images (C) are demonstrated for the localization of Dcx (green, C-i), c-Fos (red, C-ii; or green, C-v), and BrdU-labeled (red, C-iv) immunofluorescence positive cells. Merged images showed the co-localization of Dcx and c-Fos (C-iii), as well as BrdU and c-Fos (C-vi) in the hippocampal DG region (scale bar: 40 μm). Indication: *, significant difference from the sham rats, (p < 0.05).