Fig. 2.
Genetic-background-, gender- and age-dependent differences in the motor phenotype of Cln3−/− and Cln3Δex7/8-knock-in mice – climbing down a vertical pole. The ability of the mice to climb down a vertical pole was examined. One-, 3- and 6-month-old Cln3−/− and Cln3Δex7/8-knock-in (Cln3KI) mice on two different genetic backgrounds (129S6/SvEv and C57BL/6J), as well as 129S6/SvEv and C57BL/6J WT mice, were tested. Mice were placed, head downwards, on top of a 59-cm-long all-thread metal pole, and the time until the mice climbed down to the base of the pole was measured in five consecutive trials. Data are plotted as the total time of the five trials. Columns and bars represent mean±s.e.m. (n=10–12). The same mice were tested at 1, 3 and 6 months of age. Statistical significance was determined by 1-way ANOVA with Bonferroni’s post-test for multiple comparisons. Asterisks indicate the significant difference between 129S6/SvEv and C57BL/6J WT females at 6 months of age: **P<0.01.