Fig. 5.
Genetic-background-, gender- and age-specific differences in the depressive-like behavior of Cln3−/− and Cln3Δex7/8-knock-in mice. The depressive-like behavior of 1-, 3- and 6-month-old Cln3−/− and Cln3Δex7/8-knock-in (Cln3KI) mice on two different genetic backgrounds (129S6/SvEv and C57BL/6J) was assessed by the tail suspension test. 129S6/SvEv and C57BL/6J WT mice served as controls. Mice were suspended 45 cm above table level, and the duration of immobility was measured in 1-minute bins for 6 minutes. The graphs show the total time of immobility in the 6 minutes of the test; columns and bars represent mean±s.e.m. (n=10–12). The same mice were tested at 1, 3 and 6 months of age. Statistical significance was determined by 1-way ANOVA with Bonferroni’s post-test for multiple comparisons. Asterisks indicate significant differences between 129S6/SvEv and C57BL/6J WT mice, males versus males, and females versus females: *P<0.05 and ****P<0.0001.