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. 2015 Mar 17;15:135. doi: 10.1186/s12885-015-1138-8

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© Gambaro et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of IGFBP7-containing conditioned media on EOC cell lines. A, Immunoblot analysis monitoring IGFBP7 protein levels in the CM from EOC cell lines. Recombinant IGFBP7 protein was used as a positive control and two different exposures are shown (10 and 60 seconds). B, Spheroid formation assay of OV90 neo^r and TOV112D cell lines cultured in IGFBP7-containing CM (TOV21G and TOV81D) or control CM (TOV112D). Images are shown at × 20 magnification. C, Wound healing assay with OV90 neo^r and TOV112D cultured in IGFBP7-containing CM (TOV21G and TOV81D) or control CM (TOV112D) observed over a period of 6 days. Images are shown at × 10 magnification.