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. 2015 Mar 21;16(1):220. doi: 10.1186/s12864-015-1374-y

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© Streva et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Description of SIMPLE. (A) Randomly sheared genomic DNA (black line) is subjected to a single round of primer extension using a primer specific to the L1 5’ UTR (blue arrow). Only DNA fragments containing a full length L1 element (large red arrow) will undergo linear extension from the L1-specific primer. DNA fragments containing no L1 sequence or truncated L1 copies (bottom left and right respectively) will not be extended. Following primer extension, a duplex “t-linker” (orange) is ligated to L1-primer extended ends. (B) Following ligation of duplex linkers, adapter sequences needed for Illumina sequencing (purple bars) are added by nested PCR to only those fragments anchored by an L1 extension event. (C) Following gel based size fractionation, SIMPLE libraries are run on an agarose gel and analyzed by Agilent BioAnalyzer to confirm size and library quality.